Crystallization and preliminary crystallographic analysis of the DNA gyrase B protein from B. stearothermophilus.
نویسندگان
چکیده
DNA gyrase B (GyrB) from B. stearothermophilus has been crystallized in the presence of the non-hydrolyzable ATP analogue, 5'-adenylyl-beta-gamma-imidodiphosphate (ADPNP), by the dialysis method. A complete native data set to 3.7 A has been collected from crystals which belonged to the cubic space group I23 with unit-cell dimension a = 250.6 A. Self-rotation function analysis indicates the position of a molecular twofold axis. Low-resolution data sets of a thimerosal and a selenomethionine derivative have also been analysed. The heavy-atom positions are consistent with one dimer in the asymmetric unit.
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عنوان ژورنال:
- Acta crystallographica. Section D, Biological crystallography
دوره 52 Pt 6 شماره
صفحات -
تاریخ انتشار 1996